Temporary

Part:BBa_K323164:Design

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-21)


VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
    Illegal NheI site found at 2568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 6132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5657
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

a


Source

a

References